MineOS Turnkey is Debian. Rather than pull your hair out about the inability for Rufus to created a bootable ISO from the Turnkey ISO is probably not the best use of your efforts.
Ubuntu is a slightly-modified debian, but for all intents and purposes, it is wholly compatible. But if you’re still not interested in Ubuntu, you can just install Debian (one that likely will install from Rufus-made USB sticks) and then use the convenience installer script linked on every wiki page for each distro.
- Using an existing setup, execute the convenience installer script via gist (wget-friendly link)
I think that getting held up on the ISO/USB process will be needlessly frustrating in comparison to the installation methods otherwise available to put the webui on Linux.